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DNA repair pathways and the effect of radiotherapy in - DiVA

Ku70/80 was incubated with PARP-1 in the presence of sonicated DNA and NAD + (lanes 1 and 3) or buffer only (lanes 2 and 4). Cell Reports Article PAXX Is an Accessory c-NHEJ Factor that Associates with Ku70 and Has Overlapping Functions with XLF Satish K. Tadi,1 Carine Tellier-Lebe`gue,2 Cle´ment Nemoz,2 Pascal Drevet,2 Ste´phane Audebert,1 Sunetra Roy,3,4 1 Dec 2008 The NHEJ pathway can be inactivated by targeting the Ku70 or Ku80 Phylogenetic tree of KU70 and KU80 fungal proteins whose function in 14 Sep 2020 of the Ku70/80 heterodimer in vitro. We further defined the function of the Ku70 and Ku80 C-terminal domains in DNA end binding and They also have significantly diminished levels of Ku80 protein. Therefore, Ku70's role in DNA repair may be linked to the known biochemical features of Ku, InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures 5 Mar 2021 Plays a role in the regulation of DNA virus-mediated innate immune response by Heterodimer composed of XRCC5/Ku80 and XRCC6/Ku70.

Ku70 and ku80 function

Ku70 is an evolutionarily conserved protein that has functions in DNA repair and maintenance [96]. It is a heterodimeric protein made up of Ku70 and Ku80 [97] . We (unpublished data) and others [43] had evaluated and shown that proteins involved in DNA repair including DNA-PK, p53, ATM and Ku70 had no effect on the type-I-IFN response induced by cytosolic dsDNA stimulation. There was no correlation between the transcript levels of Ku80 and LUC luminescence derived from T‐DNA stable transformation in KD‐OsKu80 rice calli (Figs 1b, 2d,e). It has been shown that the Ku70/80 protein functions as a heterodimer to repair DSBs, with the two constituents stabilizing each other (Smider et al., 1994).

DNA repair pathways and the effect of radiotherapy in - DiVA

These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background. First, either Ku70 or Ku80 functions outside the Ku heterodimer such that deletion of one is not identical to deletion of the other. Second, divergent genetic backgrounds or environments inﬂuence the phenotype.

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They show that PAXX’s function overlaps with c-NHEJ factor XLF, explaining why its ablation has only a mild effect on NHEJ. Ku70 is the 70 kDa subunit of the lupus Ku autoantigen. The lupus Ku autoantigen was identified in individuals with systemic lupus erythematosus (SLE) and related disorders. The Ku antigen is a heterodimer of Ku70 and Ku80. The Ku70/Ku80 complex functions as a single-stranded DNA-dependent ATP-dependent helicase. Function Together, Ku70 and Ku80 make up the Ku heterodimer , which binds to DNA double-strand break ends and is required for the non-homologous end joining (NHEJ) pathway of DNA repair .

Microtubules.Orthoptera.RNAi.Spindleassembly checkpoint Abbreviations Ku70 and Ku80. Ku70 and Ku80 form a heterodimer, Ku, which possesses DNA end-binding activity (Mimori and Hardin, 1986 ). Purified Ku protein was found to promote the association of two DNA molecules in vitro; thus, it was proposed to possess end bridging or alignment activity ( … We conclude that Ku70 and Ku80 may have functions in V (D)J recombination and DNA repair that are independent of DNA-PKcs. might function as part of a telomeric length sensing system protecting chromosomal termini from nucleolytic attack, as was shown in yeast (Boulton and Jackson, 1996).
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We (unpublished data) and others [43] had evaluated and shown that proteins involved in DNA repair including DNA-PK, p53, ATM and Ku70 had no effect on the type-I-IFN response induced by cytosolic dsDNA stimulation. Heterodimers of the 70 and 80 kDa Ku autoantigens (Ku70 and Ku80) activate the DNA‐dependent protein kinase (DNA‐PK). Mutations in any of the three subunits of this protein kinase (Ku70, Ku80 and DNA‐PKcs) lead to sensitivity to ionizing radiation (IR) and to DNA double‐strand breaks, and V(D)J recombination product formation defects.

Ku80 works as a heterodimer with Ku70. Heterodimers of the 70 and 80 kDa Ku autoantigens (Ku70 and Ku80) activate the DNA‐dependent protein kinase (DNA‐PK). Mutations in any of the three subunits of this protein kinase (Ku70, Ku80 and DNA‐PKcs) lead to sensitivity to ionizing radiation (IR) and to DNA double‐strand breaks, and V(D)J recombination product formation defects. also been suggested that Ku80 has a Ku70-independent DNA DSB repair function, in addition to the one depen-dent on Ku70 (Koike and Koike 2005).
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Purified Ku protein was found to promote the association of two DNA molecules in vitro; thus, it was proposed to possess end bridging or alignment activity ( … We conclude that Ku70 and Ku80 may have functions in V (D)J recombination and DNA repair that are independent of DNA-PKcs.

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Preclinical evaluation of (111)In-DTPA-INCA-X anti-Ku70/Ku80 monoclonal av P Umate · 2011 · Citerat av 90 — The role of these motifs in ATP-binding, ATP-hydrolysis (ATPase), RNA A total of 31 DNA helicases (like RecQ members, KU70, MCM Protection of Kidney Function with Human Antioxidation Protein Preclinical evaluation of (111)In-DTPA-INCA-X anti-Ku70/Ku80 monoclonal antibody in 22 jan. 2564 BE — CRISPR/Cas9-mediated loss of FGF5 function increases wool staple we suppressed the NHEJ key molecules KU70, KU80 or DNA ligase IV By agreement with the company, the product's function will not be exposed in the report. of CUX1 with some of these proteins: REV1, PARP1 and Ku70/Ku80. In this thesis, we have studied factors that affect tumour cell resistance to ionising radiation.

In Complex: Ku70:Ku80 complex Macromolecular complex annotations are imported from the Complex Portal . These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background. First, either Ku70 or Ku80 functions outside the Ku heterodimer such that deletion of one is not identical to deletion of the other. Second, divergent genetic backgrounds or environments inﬂuence the phenotype.